Honeybee Queens tooting and quacking is communication with the workers.
Really, would I lie to you,
listen.
When the new queens are ready to be released from their brood cells they quack.
The reigning queen toots as long as she is there.
When she takes off to start a new hive the tooting stops and the workers release a
quacking queen who immediately starts tooting to signal the workers to not release
another queen unless they want a death match.