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Your installation will have drivers specifically for the motherboard, as well as the rest of the hardware. If you change motherboards, especially to something radically different, there is a chance it won't boot.
But it's worth a try and I must add that I've swapped MBs several times over the life of my current installation.
There are procedures out there to remove the old drivers BEFORE beginning the install of the new board. That way Win2K boots to something more "generic" and you have a chance to add the right drivers.
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